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1 Astrocyte Medium (AM) Catalog # 1801 Product Description Astrocyte Medium ), when used with Astrocyte Growth Supplement (AGS , Cat # 1852) and (AM 10 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal in vitro . It is a sterile, liquid medium which contains essential and non -essential amino astrocytes acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and 2% ). The medium is HEPES and bicarbonate buffered a low concentration of fetal bovine serum ( and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO /95% air. 2 The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced in vitro . nutritional environment that selectively supports the growth of normal astrocytes Components consists of 500 ml of basal medium, 10 ml of FBS (Cat. # AM 0010), 5 ml of Astrocyte Growth Supplement (AGS , Cat. #1852) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, AGS and P/S are not pre -mixed in AM ; they must be added separately to make the complete AM . Product Use in is for research use only. It is not approved for human or animal use, or for application in AM vitro diagnostic procedures. Storage o o , FBS and P/S solution at - 20 C and the AGS C. Protect from light. Store the basal medium at 4 Shipping Basal medium: room temperature. Supplements: dry ice. Instructions for Use o C. Gently tilt the tubes several times to ensure the , FBS and P/S solution at 37 Thaw AGS contents are completely mixed before adding to the medium. Spra y the medium bottle and tubes with 70% ethanol, and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior threads with fingers. Add AGS , FBS and P/S solution to the medium and mix well. S ince several components are light -labile, the medium should not be exposed to light o C water bath prior to use. for extended periods. We do not recommend warming medium in a 37 o C, the reconstituted medium is stable for one month. When stored in the dark at 4 Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate preca utions when handling it , including the wearing of protective clothing and eyewear. Dispose of properly.

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